iPS Cell Gene-Editing Survey Question Title * 1. Email address: Question Title * 2. Contact Information: Name * Company * Title/ Position Area of Research Question Title * 3. Do you use gene-edited iPS cells in your research? Yes, I am currently using gene-edited iPS cells Yes, I am currently using gene-edited differentiated cells No, I don’t currently use but I am planning to use them within the next 6 months Gene-edited iPS cells are not applicable for my research Other (please specify) Question Title * 4. If yes, in what application would you be using the gene-edited iPS cells? (Choose one option) Basic/disease biology Drug discovery ADME/Tox Other (please specify) Question Title * 5. What genetic change are you interested in introducing in iPS cells? (Choose max two options) Knockout by frameshift mutation Deletion Point Mutation Tag Reporter gene Other (please specify) Question Title * 6. How did you/are you planning to source custom gene-edited iPS cells? I did /I am planning to do gene-editing myself From an external vendor From an academic core facility From a collaborator Other (please specify) Question Title * 7. If you did/will do gene-editing yourself, what are the biggest challenges that you have faced so far? Question Title * 8. If you ordered/will order custom gene-edited iPS cell lines from an external vendor, what is important to you when you choose the vendor? (Choose max two options) Timelines Reputation Price Risk management Intellectual property Other (please specify) Question Title * 9. If you wanted to outsource gene-editing of iPS cells, would you prefer final deliverable to be Gene-edited iPS cells Fully differentiated cells Question Title * 10. If you are interested in fully differentiated cells, what cell type(s) are you interested in? Neuronal stem cells Cortical neurons Motor neurons Cardiomyocytes Hepatocytes Pancreatic beta cells Lung bronchial cells Other (please specify) Question Title * 11. Knockout gene-editing project by introducing frameshift mutations into both alleles includes Full characterization and optimization of cell line and reagent design One round of targeting including screening of up to 100 clones Genetic validation on gDNA level and alkaline phosphatase staining for assessment of pluripotency Delivery of at least one clone (2 vials/clone) At what price would you consider this project too expensive? (USD) At what price would you consider the project to be priced so low that you would feel that quality couldn’t be very good? (USD) At what price the project is starting to get expensive, but you still might consider it? (USD) At what price would you consider the product to be a great value for the money? (USD) Question Title * 12. I would like to participate in the prize draw Yes No Terms and conditionsOnly participants who have answered all the questions by 31 August 2016 will participate in a prize draw.Participants working for a company providing gene-editing services on a commercial basis are not eligible for the survey.The knockout gene-editing project will be performed in an iPSC line provided by the customer.The project will not be initiated before the iPSC provided have been certified to be mycoplasma-free.The knockout will be achieved by introducing frameshift mutations resulting from NHEJ into both alleles. The project includes the following: Full characterization and optimization of cell line, reagent design (screening of 5 sgRNAs), one round of targeting including screening of up to 100 clones, genetic validation on gDNA level, mycoplasma Hoechst and culture isolation testing and alkaline phosphatase staining for assessment of pluripotency.Knockout project will include one targeting event. Horizon Discovery will use its best efforts to deliver the desired genotype, however we cannot guarantee delivery for the cell line. Note that targeting phase will not be repeated in case the requested genetic modification is lethal or desired genotype has not been recovered during the first round of targeting.The cell line is for internal R&D use only. For clarity, the commercial use is specifically excluded. Submit Questionnaire